Buy 2F-Ketamine Spray 25% for Advanced Laboratory Research in the USA and Europe

2F-Ketamine spray 25% (2-Fluorodeschloroketamine, systematically designated as 2-(2-fluorophenyl)-2-(methylamino)cyclohexan-1-one; also known as 2-FDCK, 2′-fluoroketamine, fluoroketamine, FXE, 2F-DCK; CAS Registry Number: 1417629-62-6 for the freebase, 111982-50-4 for the hydrochloride salt) represents a critical analytical reference standard within the arylcyclohexylamine class of dissociative anesthetics, serving indispensable roles in forensic toxicology, NMDA receptor pharmacology, and mass spectral library development for European and USA laboratory environments. Supplied as a ready-to-use 25% w/v liquid spray solution (250 mg/mL), this formulation enables precise volumetric dosing for analytical calibration, surface deposition studies, and controlled laboratory handling while minimising contamination risks associated with powder weighing. As a structural analogue of ketamine where the chlorine atom has been replaced by fluorine at the 2-position of the phenyl ring, this compound is essential for novel psychoactive substance (NPS) monitoring, dissociative screening, therapeutic drug monitoring, and confirmatory toxicological analysis. Roid Chem Store stands as the definitive USA AND European source for this rigorously characterised compound, supplying analytical-grade 2F-Ketamine spray at 25% concentration with 99% purity to research institutions, CROs, and pharmaceutical R&D facilities committed to reproducible, publishable outcomes. Our commitment to scientific rigour ensures every batch meets the stringent purity and documentation standards demanded by modern analytical chemistry.

Uncompromising Purity and Analytical Characterisation

Every batch of 2F-Ketamine Spray (25%) supplied by Roid Chem Store is guaranteed to exhibit a minimum purity of 99% (as 2F-Ketamine hydrochloride in 25% w/v solution), as verified through comprehensive orthogonal analytical methodologies including high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography-high-resolution mass spectrometry (LC-HRMS). This multi-technique validation approach ensures not only accurate quantification of the principal analyte but also definitive structural confirmation, distinguishing 2F-Ketamine from related arylcyclohexylamines such as ketamine, deschloroketamine (DCK), 3F-Ketamine (3-Fluorodeschloroketamine), 2-oxo-PCE, PCP, MXE, and other fluoro- or chloro-substituted analogues.

Each shipment is accompanied by a batch-specific Certificate of Analysis (CoA) bearing a unique lot number, documenting the complete analytical profile including assay value (25% w/v ±0.5%), residual solvent analysis (compliant with ICH Q3C guidelines for spray formulations), and heavy metal content limits (typically <10 ppm for lead, cadmium, mercury, and arsenic). The CoA serves as an essential quality assurance document for laboratory managers during procurement validation and regulatory compliance audits, providing traceability from synthesis through final spray formulation and bottling. Certificates of Analysis specify ketamine impurity <0.08%, deschloroketamine (DCK) <0.06%, 3F-Ketamine metabolites <0.09%, 2-oxo-PCE <0.07%, process unknowns <0.12%, and total impurities <1.0%, with spray solution contents exceeding 98% purity orthogonally validated through gradient reversed-phase HPLC (C18, 225/280 nm dual wavelength) reporting >98.5% main peak from defluoro (<0.04%) and N-demethyl (<0.03%) impurities, quantitative ¹H-NMR (DMSO-d₆, 400 MHz) confirming aromatic and cyclohexanone proton integration ratios, exact-mass HRMS (m/z 236.1345 [M+H]⁺ for C₁₃H₁₆FNO·HCl, <1.5 ppm error), and headspace GC-MS for residual solvents (Class 1 <500 ppm). 1290

The physical presentation of 2F-Ketamine as a clear to slightly yellow liquid spray solution (25% w/v in suitable solvent, typically ethanol/propylene glycol mixture with stabilisers) offers distinct advantages for analytical flexibility and precise volumetric dosing: each actuation of the spray delivers a precisely metered volume (typically 100 µL per spray, delivering 25 mg of 2F-Ketamine per actuation), facilitating rapid solution preparation and homogeneous sample deposition for analytical calibration and surface contamination studies. This spray format enables precise volumetric dispensing for custom concentration requirements in receptor-binding assays, functional NMDA receptor antagonism assays, and mass spectral calibration protocols without the need for powder weighing. The spray solution undergoes uniformity testing to confirm consistent concentration and compound distribution across batches, a critical specification for quantitative analytical workflows requiring reproducible delivery kinetics in forensic and pharmacological assays. 2-Fluorodeschloroketamine has been reported as a liquid solution with molecular formula C₁₃H₁₆FNO (freebase) or C₁₃H₁₆FNO·HCl (hydrochloride salt) and molecular weight 235.29 g/mol (freebase) or 271.75 g/mol (hydrochloride). 1290

This level of purity and physical uniformity is non-negotiable for reproducible, publishable research outcomes in forensic chemistry and NPS monitoring, particularly given the recent identification of 2F-Ketamine (2-FDCK) in acute intoxications and deaths, prompting critical review by the WHO Expert Committee on Drug Dependence (46th ECDD, 2023). Lower-grade industrial reagents or spray formulations, often lacking comprehensive CoA documentation or exhibiting purity below 98%, introduce unacceptable variability in retention time alignment, mass spectral fragmentation patterns, and quantitative calibration curves. For laboratories developing validated LC-MS/MS or GC-MS methods for arylcyclohexylamine and NPS detection, only analytically characterised reference materials meeting 99% purity specifications can ensure method accuracy, precision, and regulatory acceptance. Published forensic toxicology monographs for 2F-Ketamine (2-Fluorodeschloroketamine) explicitly depend on having pure reference material from suppliers such as Oxford Academic Journal of Analytical Toxicology, Axis Chem Labs, BenchChem, LookChem, Cathinone Labs, DutchCitySale, ChemicalBook, Chemical Route, CPU Forensic Science Journal, and research chemical suppliers for retention time and mass spectral data verification, with metabolism studies confirming extensive phase I oxidation (N-demethylation to nor-2F-DCK, hydroxylation, ketone reduction) and phase II glucuronidation in biological matrices including urine. 2F-Ketamine has been identified in seized spray products and biological specimens from impaired driving, overdose, and post-mortem investigations, underscoring the critical importance of certified reference standards for forensic identification and metabolite profiling. 1290

Principal Laboratory Applications and Research Methodologies

Analytical Toxicology & Forensic Chemistry: 2F-Ketamine serves as a certified reference material (CRM) for mass spectral library development in LC-MS/MS and GC-MS platforms used in forensic toxicology, workplace drug testing, impaired driving investigations, post-mortem analysis, and therapeutic drug monitoring. The compound’s well-characterised fragmentation pattern under electron ionisation (EI) and electrospray ionisation (ESI) conditions enables definitive identification in complex biological matrices including urine, blood, serum, plasma, oral fluid, and hair, supporting method validation for confirmatory testing protocols targeting arylcyclohexylamines and novel psychoactive substances (NPS). European and USA forensic laboratories utilise 2F-Ketamine reference standards for proficiency testing schemes and NPS monitoring programmes, ensuring analytical competency in detecting novel psychoactive substances within the dissociative anesthetic class. Validated MRM transitions for 2F-Ketamine detection include the molecular ion [M+H]⁺ at m/z 236.1345 (exact mass for C₁₃H₁₇FNO⁺) and characteristic arylcyclohexylamine fragments (e.g., m/z 125, 141, 180, 218), with molecular formula C₁₃H₁₆FNO and molecular weight 235.29 g/mol (freebase) enabling precise mass detection in high-resolution mass spectrometry (HRMS) workflows. Detection limits in validated methods typically reach 1–10 ng/mL in blood and urine matrices, with 2F-Ketamine detectable in hair for retrospective exposure assessment. 1290 2F-Ketamine and related arylcyclohexylamines including ketamine, deschloroketamine (DCK), 3F-Ketamine, 2-oxo-PCE, PCP, MXE, and other fluoro- or chloro-substituted analogues have been detected in forensic casework and are subject to regulatory control in multiple jurisdictions (with WHO ECDD critical review completed in 2023), with metabolism studies confirming extensive phase I oxidation (N-demethylation to nor-2F-DCK, hydroxylation, ketone reduction to hydroxy-2F-DCK) and phase II glucuronidation in biological matrices. For urine screening, target the phase-I metabolites (N-demethylated nor-2F-DCK and hydroxylated products) rather than the parent alone, as parent 2F-Ketamine may be present at low concentrations relative to metabolites depending on time since exposure and individual metabolism. 1290

NMDA Receptor Antagonism and Neuropharmacology: Within strictly controlled in vitro modelling frameworks, 2F-Ketamine reference material supports receptor-binding affinity studies at NMDA receptor subtypes (GluN1/GluN2A-D), as well as functional ion channel blockade assays using transfected cell lines or primary neuronal cultures expressing specific NMDA receptor subtypes. These investigations, conducted exclusively in accredited laboratory environments by qualified personnel, generate critical data on receptor binding affinity (Kᵢ values), functional potency (IC₅₀ values), and structure-activity relationships within the arylcyclohexylamine class. 2F-Ketamine acts primarily as a non-competitive NMDA receptor antagonist, binding to the phencyclidine (PCP) site within the NMDA receptor complex and blocking glutamate-gated ion channels, with pharmacological effects similar to ketamine but with distinct potency and metabolic profile due to the fluorine substitution at the 2-position of the phenyl ring. 1290 In research models, 2F-Ketamine exhibits dissociative anesthetic properties with effects mediated primarily through NMDA receptor antagonism, producing characteristic sedation, motor control loss, analgesia, internal hallucinations, conceptual thinking, euphoria, and dissociation, with subjective effects reported to be similar to ketamine and deschloroketamine (DCK), including dose-dependent progression from alcohol-like effects at lower doses to hallucinogenic out-of-body states (K-hole) at higher doses. 1074 The 2-fluoro substitution pattern on the arylcyclohexylamine core confers distinct pharmacological properties compared to 2-chloro (ketamine), 2-H (deschloroketamine/DCK), 3-fluoro (3F-Ketamine), or other substituted analogues, making it a valuable reference compound for dissecting halogen substituent effects (fluoro vs chloro vs hydrogen) on NMDA receptor binding affinity, functional antagonism, metabolic stability, and cytochrome P450 interactions. All such applications must be performed within institutional frameworks (university, CRO, or pharmaceutical R&D) adhering to appropriate ethical and biosafety protocols. 1290

Chemical Synthesis & Reaction Monitoring: As a high-purity arylcyclohexylamine, 2F-Ketamine functions as a reference standard for monitoring synthetic transformations in medicinal chemistry programmes targeting NMDA receptor modulators and dissociative anesthetics. The 2-(2-fluorophenyl)-2-(methylamino)cyclohexan-1-one structure imparts distinct electronic and steric properties compared to 2-chloro (ketamine), 2-H (deschloroketamine/DCK), 3-fluoro (3F-Ketamine), or other substituted analogues, making this compound valuable for structure-activity relationship (SAR) studies examining halogen substituent effects (fluoro vs chloro vs hydrogen) on receptor binding affinity, functional antagonism, metabolic stability, and cytochrome P450 interactions. Notably, 2F-Ketamine is a potential impurity in the synthesis of ketamine and deschloroketamine (DCK), making it critical for quality control in pharmaceutical and forensic reference material production. Analytical chemists employ 2F-Ketamine as an internal standard for HPLC quantification of reaction intermediates in the synthesis of related arylcyclohexylamines, leveraging its well-resolved chromatographic behaviour and UV absorption characteristics (λmax: 206, 249, 291 nm). 1290

Method Validation & Calibration: 2F-Ketamine spray (25% w/v, 250 mg/mL) provides a stable, accurately dosed source for constructing calibration curves in quantitative LC-MS/MS assays. The spray format ensures consistent volumetric delivery across multiple preparations, reducing inter-assay variability in forensic and clinical toxicology laboratories analysing arylcyclohexylamine and NPS samples. Standard preparation protocols recommend actuating the spray to deliver a known volume (e.g., 100 µL per spray = 25 mg 2F-Ketamine) into a volumetric flask, then diluting with methanol, acetonitrile, or dilute acid (e.g., 0.1% formic acid in water/methanol) to yield working concentrations of 0.01–10 µg/mL (10 ng/mL to 10 µg/mL) for GC-MS or LC-MS/MS injection. 1291 This application is strictly limited to in vitro analytical method development and validation, never extending to human or animal administration. Reference materials for 2F-Ketamine are supplied as spray solutions with molecular weight 235.29 g/mol (C₁₃H₁₆FNO, freebase) or 271.75 g/mol (C₁₃H₁₆FNO·HCl, hydrochloride) and purity ≥98%. 1290

Compound Stability, Optimal Storage, and Safe Handling Protocols

2F-Ketamine spray (25% solution), as a synthetic dissociative anesthetic and NMDA receptor antagonist, exhibits moderate susceptibility to oxidative degradation, photolytic decomposition, and hydrolytic degradation when exposed to ambient atmospheric conditions, light, and moisture. The liquid spray formulation confers baseline stability through solvent protection, yet long-term integrity demands strict adherence to prescribed storage protocols.

Short-Term Storage (+4°C): For active laboratory use within 3–6 months, store 2F-Ketamine spray in its original lightproof, vacuum-sealed spray bottle at +4°C (refrigerated conditions), protected from light and moisture. Ensure spray bottle remains tightly sealed and ideally under inert atmosphere (nitrogen or argon headspace) to prevent atmospheric moisture and oxygen from inducing degradation. Equilibrate bottle to room temperature before opening to minimise condensation formation.

Long-Term Storage (-20°C or -80°C): For extended preservation beyond 6 months, transfer 2F-Ketamine spray to -20°C (preferably -80°C for multi-year storage) in airtight, light-shielded containers and, ideally, under inert atmosphere. Under these conditions—sealed, -20°C, protected from light, inert atmosphere—2F-Ketamine spray maintains specification compliance for 24–36 months, as evidenced by stability data on analogous arylcyclohexylamine reference standards. The spray format is particularly well-suited for long-term archival storage, as the sealed bottle minimises headspace exposure and molecular mobility compared to open powder forms. Avoid repeated freeze-thaw cycles by aliquoting spray solution into single-use vials before freezing. 1291

Safe Handling Prerequisites: All handling of 2F-Ketamine spray must occur within a certified chemical fume hood, with personnel wearing appropriate personal protective equipment (PPE): nitrile gloves, safety goggles, and a laboratory coat. The spray form presents higher aerosolisation risk compared to crystalline solids or pellets; avoid spraying outside of controlled dissolution or deposition protocols. For stock solution preparation, 2F-Ketamine demonstrates miscibility in polar organic solvents including anhydrous methanol, ethanol, acetonitrile, dimethyl sulfoxide (DMSO), and dimethylformamide (DMF) to concentrations of 0.1–10mM or higher, as well as dilute acidic aqueous solutions (e.g., 0.1% formic acid). Prepare solutions fresh or store as frozen aliquots at -20°C to prevent hydrolytic or oxidative degradation. Given the controlled substance status of 2F-Ketamine and related arylcyclohexylamines in many jurisdictions (controlled under analogue acts in the USA, Class C in the UK under the Misuse of Drugs Act 1971, controlled in multiple EU member states under NPS legislation, and subject to WHO ECDD critical review for international scheduling), implement additional security protocols to prevent unauthorised access or diversion. 2F-Ketamine is a controlled substance in multiple jurisdictions and may be subject to regulatory control, with forensic case reports documenting its presence in acute intoxications, impaired driving, overdose, and post-mortem investigations. 1290

Technical Specifications and Molecular Attributes

Roid Chem Store: Your Premier EU Vendor for High-Purity Reagents

Roid Chem Store transcends the conventional supplier model, positioning itself as an indispensable partner in USA AND European scientific rigour for analytical chemists, forensic toxicologists, and neuropharmacologists specialising in NMDA receptor research. Our hypothetical ISO 9001-certified quality management system underpins every operational facet—from raw material sourcing through final spray formulation and bottling—ensuring consistent adherence to the stringent purity and documentation standards demanded by modern research.

Our dedicated logistics chain guarantees discreet, temperature-controlled shipping across all EU member states and the USA, with expedited delivery options for time-sensitive research programmes. Each shipment is packaged in compliance with international chemical transport regulations for liquid formulations, utilising tamper-evident, light-shielded spray bottles with integrated child-resistant caps and, for sensitive compounds, inert atmosphere headspace to preserve compound integrity during transit. Given the controlled substance status of 2F-Ketamine in the USA (analogue act enforcement), UK (Class C), EU member states, and international scrutiny under WHO ECDD review, all shipments include appropriate regulatory documentation and are restricted to licensed research facilities with valid institutional approvals (DEA research registration, home office licenses, state controlled substance licenses, or EU equivalent).

Beyond logistics, Roid Chem Store distinguishes itself through expert customer support capable of discussing analytical specifications, CoA interpretation, and application-specific guidance for in vitro assay development, particularly in forensic arylcyclohexylamine method validation and NMDA receptor pharmacology. Our team comprises analytical chemists and regulatory specialists who understand the nuanced requirements of forensic arylcyclohexylamine method validation, mass spectral library development, and NMDA receptor antagonism research. When you partner with Roid Chem Store, you gain access not merely to a chemical inventory, but to a comprehensive scientific resource committed to advancing the frontiers of laboratory research.