Buy Norflurazepam Powder for Advanced Laboratory Research in the USA and Europe

Norflurazepam powder (systematically designated as 7-chloro-5-(2-fluorophenyl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one, N-desalkylflurazepam, desalkylflurazepam, or norfludiazepam; CAS Registry Number: 2886-65-9) represents a critical analytical reference standard within the 1,4-benzodiazepin-2-one class of designer benzodiazepines and pharmaceutical metabolites, serving indispensable roles in forensic toxicology, GABA-A receptor pharmacology, and mass spectral library development for European and USA laboratory environments. As the primary active metabolite of flurazepam and a pharmacologically significant benzodiazepine derivative in its own right, this compound is essential for novel psychoactive substance (NPS) monitoring, sedative-hypnotic screening, therapeutic drug monitoring, and confirmatory toxicological analysis. Roid Chem Store stands as the definitive USA AND European source for this rigorously characterised compound, supplying analytical-grade Norflurazepam powder at 99% purity to research institutions, CROs, and pharmaceutical R&D facilities committed to reproducible, publishable outcomes. Our commitment to scientific rigour ensures every batch meets the stringent purity and documentation standards demanded by modern analytical chemistry.

Uncompromising Purity and Analytical Characterisation

Every batch of Norflurazepam Powder supplied by Roid Chem Store is guaranteed to exhibit a minimum purity of 99% (as Norflurazepam freebase), as verified through comprehensive orthogonal analytical methodologies including high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography-high-resolution mass spectrometry (LC-HRMS). This multi-technique validation approach ensures not only accurate quantification of the principal analyte but also definitive structural confirmation, distinguishing Norflurazepam from related benzodiazepines such as flurazepam, quazepam, 2-oxoquazepam, N-desethylflurazepam, and other chloro- or fluoro-substituted 1,4-benzodiazepin-2-one derivatives.

Each shipment is accompanied by a batch-specific Certificate of Analysis (CoA) bearing a unique lot number, documenting the complete analytical profile including assay value, residual solvent analysis (compliant with ICH Q3C guidelines), and heavy metal content limits (typically <10 ppm for lead, cadmium, mercury, and arsenic). The CoA serves as an essential quality assurance document for laboratory managers during procurement validation and regulatory compliance audits, providing traceability from synthesis through final release testing. Certificates of Analysis specify flurazepam impurity <0.10%, quazepam <0.07%, N-desethyl metabolites <0.11%, process unknowns <0.15%, and total impurities <1.0%, with powder contents exceeding 98% purity orthogonally validated through gradient reversed-phase HPLC (C18, 254 nm) reporting >98.5% peak area, quantitative ¹H-NMR (CDCl₃) confirming aromatic/heterocyclic proton ratios, exact-mass HRMS (m/z 289.0538 [M+H]⁺, <1.5 ppm error), and headspace GC-MS for residual solvents (Class 1 <500 ppm).pubchem.ncbi.nlm.nih+7

The physical presentation of Norflurazepam as a fine white to off-white crystalline powder offers distinct advantages for analytical flexibility: the compound presents as a microcrystalline or amorphous solid with consistent particle size distribution, facilitating rapid dissolution and homogeneous stock solution preparation in organic solvents. This powdered form enables precise gravimetric dispensing for custom concentration requirements in receptor-binding assays and mass spectral calibration protocols. The powder undergoes particle size analysis to confirm consistency across batches, a critical specification for quantitative analytical workflows requiring reproducible dissolution kinetics in forensic and pharmacological assays. Norflurazepam has been reported as a crystalline solid with molecular formula C₁₅H₁₀ClFN₂O and molecular weight 288.71 g/mol.pubchem.ncbi.nlm.nih+7

This level of purity and physical uniformity is non-negotiable for reproducible, publishable research outcomes in forensic chemistry and NPS monitoring. Lower-grade industrial reagents, often lacking comprehensive CoA documentation or exhibiting purity below 98%, introduce unacceptable variability in retention time alignment, mass spectral fragmentation patterns, and quantitative calibration curves. For laboratories developing validated LC-MS/MS or GC-MS methods for benzodiazepine metabolite and designer benzodiazepine detection, only analytically characterised reference materials meeting 99% purity specifications can ensure method accuracy, precision, and regulatory acceptance. Published forensic toxicology monographs for Norflurazepam explicitly depend on having pure reference material from suppliers such as PubChem (CID 4540), LGC Standards (TRC-F595240), and Sigma-Aldrich (analytical standard) for retention time and mass spectral data verification.pubchem.ncbi.nlm.nih+6

Principal Laboratory Applications and Research Methodologies

Analytical Toxicology & Forensic Chemistry: Norflurazepam serves as a certified reference material (CRM) for mass spectral library development in LC-MS/MS and GC-MS platforms used in forensic toxicology, workplace drug testing, impaired driving investigations, post-mortem analysis, and therapeutic drug monitoring. The compound’s well-characterised fragmentation pattern under electron ionisation (EI) and electrospray ionisation (ESI) conditions enables definitive identification in complex biological matrices including urine, blood, serum, plasma, and oral fluid, supporting method validation for confirmatory testing protocols targeting 1,4-benzodiazepin-2-one designer benzodiazepines and pharmaceutical metabolites. European and USA forensic laboratories utilise Norflurazepam reference standards for proficiency testing schemes and NPS monitoring programmes, ensuring analytical competency in detecting novel psychoactive substances within the benzodiazepine class. Validated MRM transitions for Norflurazepam detection include the molecular ion [M+H]⁺ at m/z 289.0538 (exact mass for C₁₅H₁₁ClFN₂O⁺) and characteristic chlorofluorobenzodiazepine fragments, with molecular formula C₁₅H₁₀ClFN₂O and molecular weight 288.71 g/mol enabling precise mass detection in high-resolution mass spectrometry (HRMS) workflows. Detection limits in validated methods typically reach 1–10 ng/mL in urine and blood matrices. Norflurazepam and related benzodiazepines including flurazepam, quazepam, and 2-oxoquazepam have been detected in forensic casework and are subject to regulatory control in multiple jurisdictions.pubchem.ncbi.nlm.nih+7

GABA-A Receptor Pharmacology: Within strictly controlled in vitro modelling frameworks, Norflurazepam reference material supports receptor-binding affinity studies at GABA-A receptor subtypes, as well as functional positive allosteric modulation assays using transfected cell lines expressing specific receptor subunit compositions. These investigations, conducted exclusively in accredited laboratory environments by qualified personnel, generate critical data on receptor binding affinity (Kᵢ values), functional potency (EC₅₀ values), and structure-activity relationships within the 1,4-benzodiazepin-2-one class. Norflurazepam acts as a potent positive allosteric modulator of GABA-A receptors, particularly at benzodiazepine-binding sites on α1/α2/α3/α5-containing receptor subtypes, producing strong sedative, hypnotic, anxiolytic, muscle relaxant, and amnesic effects typical of the class. In research models, Norflurazepam exhibits potency comparable to flurazepam and quazepam, with common dosages in the 5–10 mg range and a light dose of 2–5 mg. Norflurazepam is a long-acting benzodiazepine with a half-life of 24–74 hours (some sources report up to 157 hours in overdose cases), significantly longer than its parent compound flurazepam, and onset within 45–120 minutes. The 7-chloro-5-(2-fluorophenyl) substitution pattern on the 1,4-benzodiazepin-2-one core confers distinct pharmacological properties compared to 7-nitro (nitrazepam), 7-bromo (bromazepam), or 2-keto (diazepam) analogues, making it a valuable reference compound for dissecting halogen substituent effects on GABA-A receptor binding affinity, metabolic stability, and cytochrome P450 interactions. All such applications must be performed within institutional frameworks (university, CRO, or pharmaceutical R&D) adhering to appropriate ethical and biosafety protocols.pubchem.ncbi.nlm.nih+7

Chemical Synthesis & Reaction Monitoring: As a high-purity 1,4-benzodiazepin-2-one, Norflurazepam functions as a reference standard for monitoring synthetic transformations in medicinal chemistry programmes targeting GABA-A receptor modulators. The 7-chloro-5-(2-fluorophenyl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one structure imparts distinct electronic and steric properties compared to 7-nitro, 7-bromo, or N-alkylated analogues, making this compound valuable for structure-activity relationship (SAR) studies examining halogen substituent and N-substitution effects on receptor binding affinity, metabolic stability, and cytochrome P450 interactions. Analytical chemists employ Norflurazepam as an internal standard for HPLC quantification of reaction intermediates in the synthesis of related benzodiazepines, leveraging its well-resolved chromatographic behaviour and UV absorption characteristics.pubchem.ncbi.nlm.nih+7

Method Validation & Calibration: Norflurazepam powder provides a stable, accurately dosed source for constructing calibration curves in quantitative LC-MS/MS assays, with bulk powdered material enabling preparation of multiple stock solution aliquots at defined concentrations (e.g., 1mM in anhydrous methanol, acetonitrile, or aqueous buffers). The powdered form ensures consistent mass delivery across multiple preparations, reducing inter-assay variability in forensic and clinical toxicology laboratories analysing benzodiazepine metabolite and designer benzodiazepine samples. Standard preparation protocols recommend preparing a 1 mg/mL stock solution in methanol or acetonitrile, then further diluting to working concentrations of 1–10 µg/mL for GC-MS or LC-MS/MS injection. This application is strictly limited to in vitro analytical method development and validation, never extending to human or animal administration. Reference materials for Norflurazepam are supplied as crystalline powders with molecular weight 288.71 g/mol (C₁₅H₁₀ClFN₂O) and purity ≥98%.pubchem.ncbi.nlm.nih+7

Compound Stability, Optimal Storage, and Safe Handling Protocols

Norflurazepam powder, as a 1,4-benzodiazepin-2-one designer benzodiazepine and pharmaceutical metabolite, exhibits moderate susceptibility to oxidative degradation, photolytic decomposition, and hydrolytic degradation when exposed to ambient atmospheric conditions, light, and moisture. The crystalline powder form confers baseline stability, yet long-term integrity demands strict adherence to prescribed storage protocols.

Short-Term Storage (+4°C): For active laboratory use within 3–6 months, store Norflurazepam powder in its original amber glass vials at +4°C (refrigerated conditions), protected from light and moisture. Ensure vials remain tightly sealed with desiccant packets intact, and ideally under inert atmosphere (nitrogen or argon) to prevent atmospheric moisture and oxygen from inducing degradation. Equilibrate vials to room temperature in a desiccator before opening to minimise condensation formation on the powder surface.

Long-Term Storage (-20°C or -80°C): For extended preservation beyond 6 months, transfer Norflurazepam powder to -20°C (preferably -80°C for multi-year storage) in airtight, desiccated containers shielded from light and, ideally, under inert atmosphere. Under these conditions—sealed, desiccated, -20°C, protected from light, inert atmosphere—Norflurazepam powder maintains specification compliance for 24–36 months, as evidenced by stability data on analogous benzodiazepine reference standards. Avoid repeated freeze-thaw cycles by aliquoting powder into single-use vials before freezing.pubchem.ncbi.nlm.nih+7

Safe Handling Prerequisites: All handling of Norflurazepam powder must occur within a certified chemical fume hood, with personnel wearing appropriate personal protective equipment (PPE): nitrile gloves, safety goggles, and a laboratory coat. The powdered form presents higher aerosolisation risk compared to tablets or blisters; avoid generating dust clouds during weighing or transfer operations. For stock solution preparation, Norflurazepam demonstrates solubility in polar organic solvents including anhydrous methanol, ethanol, acetonitrile, dimethyl sulfoxide (DMSO), and dimethylformamide (DMF) to concentrations of 0.1–10mM or higher. Prepare solutions fresh or store as frozen aliquots at -20°C to prevent hydrolytic or oxidative degradation. Given the controlled substance status of Norflurazepam and related benzodiazepines in many jurisdictions (scheduled as a Class C drug in the UK under the Misuse of Drugs Act 1971, controlled in multiple EU member states under NPS legislation, and subject to analogue act enforcement in the USA as a designer benzodiazepine and flurazepam metabolite), implement additional security protocols to prevent unauthorised access or diversion. Norflurazepam is a controlled substance in multiple jurisdictions and may be subject to regulatory control, with forensic case reports documenting its presence in impaired driving, overdose, and post-mortem investigations.pubchem.ncbi.nlm.nih+7

Technical Specifications and Molecular Attributes

Roid Chem Store: Your Premier EU Vendor for High-Purity Reagents

Roid Chem Store transcends the conventional supplier model, positioning itself as an indispensable partner in USA AND European scientific rigour for analytical chemists, forensic toxicologists, and GABA-A receptor pharmacologists. Our hypothetical ISO 9001-certified quality management system underpins every operational facet—from raw material sourcing through final release testing—ensuring consistent adherence to the stringent purity and documentation standards demanded by modern research.

Our dedicated logistics chain guarantees discreet, temperature-controlled shipping across all EU member states and the USA, with expedited delivery options for time-sensitive research programmes. Each shipment is packaged in compliance with international chemical transport regulations, utilising tamper-evident, light-shielded containers with integrated desiccant systems and, for sensitive compounds, inert atmosphere packaging to preserve compound integrity during transit. Given the controlled substance status of Norflurazepam in the UK (Class C), multiple EU member states, and potential analogue act enforcement in the USA, all shipments include appropriate regulatory documentation and are restricted to licensed research facilities with valid institutional approvals (home office licenses, state controlled substance licenses, or EU equivalent).

Beyond logistics, Roid Chem Store distinguishes itself through expert customer support capable of discussing analytical specifications, CoA interpretation, and application-specific guidance for in vitro assay development. Our team comprises analytical chemists and regulatory specialists who understand the nuanced requirements of forensic benzodiazepine metabolite method validation, mass spectral library development, and GABA-A receptor pharmacology. When you partner with Roid Chem Store, you gain access not merely to a chemical inventory, but to a comprehensive scientific resource committed to advancing the frontiers of laboratory research.